Xanthohumol, also known as glutaryl chalcone, is an active ingredient found in hops. Xanthohumol has been found to have a variety of anti-cancer activities, For example, Slawi Productive ska-Brych found that Xanthohumol can inhibit the growth and proliferation of laryngeal cancer cells by inhibiting the apoptosis of laryngeal cancer cells and the G1 phase. Hou found that Xanthohumol could induce apoptosis of rat glioma cells C6 by inducing mitochondrial stress and activating AIF. Seitz found that Xanthohumol had a good inhibitory effect in vitro and in vivo, and could also inhibit the liver metastasis of melanoma. Liu found that Xanthohumol could inhibit the growth of colon cancer cells in human colon cancer by inducing apoptosis of colon cancer cells, blocking cells in G2/M phase and blocking MEK/ERK pathway in colon cancer cells. Xanthohumol also has anti-inflammatory, antibacterial and lipid-lowering effects, but no one has studied the effect of xanthohumol on skin squamous cell carcinoma. Effects of xanthohumol at different concentrations on A431 cell viability. It was found that with the increase of xanthohumol concentration, cell viability decreased. At the same time, the scratch closure rate of A431 cells was significantly decreased after xanthohumol treatment, and the number of invading and migrating cells was significantly decreased. Fluorescence results showed that the number of normal cells decreased and the number of apoptotic and necrotic cells increased after xanthohumol treatment.
The JAK/STAT pathway is one of the important signaling pathways that regulate cell proliferation, differentiation, migration and apoptosis . At the same time, the JAK/STAT pathway can regulate the expression of the downstream anti-apoptotic protein Bcl-2 and cause the inhibition of apoptosis . In this study, we found that after xanthohumol acted on human squamous cell carcinoma A431 cells, the protein expressions of JAK2, STAT3 and Bcl-2 in the cells showed a decreased trend. Therefore, we speculated that the anti-xanthohumol effect on human squamous cell carcinoma A431 cells might be realized by inhibiting the expression of JAK2, STAT3 and Bcl-2 proteins.
In conclusion, xanthohumol can inhibit the growth, migration and invasion of A431 cells, and promote the apoptosis of A431 cells by inhibiting the expression of JAK2, STAT3 and Bcl-2 proteins, which provides a certain experimental basis for the clinical application of xanthohumol as a potential anti-squamous cell carcinoma drug.
Post time: Nov-23-2022
